重组金黄色葡萄球菌蛋白A(RecombinantSPA)

HPLC纯化：纯度>98%；

分子量：45-47kDa；

等电点：4.5

蛋白A是从A类Streptococci细菌中分离出来的胞壁蛋白，与与人及多种哺乳动物血清IgG分子中的Fc片段结合（包括：人、山羊、绵羊、兔、豚鼠、马、猪、猴、小鼠等）。不与狗IgG结合、不结合人IgM、IgD、和IgA。

重组金黄色葡萄球菌蛋白A(SPA)（Recomb Protein A）是大肠杆菌生产的基因工程产品，具有极高Fc段结合活性,性能稳定，易于纯化等优点。而且已经除去了与白蛋白及细胞表面结合位点，减少了交叉反应和非特异性结合。因此，它比天然蛋白G和蛋白A有更大的亲和力。可以代替二抗，广泛应用于免疫化学等领域。在亲和力、稳定性等方面很好。

蛋白A是从非致病性Bacillus中获取的通过基因工程生产的重组蛋白，其性质和重组蛋白G相似。

蛋白A主要应用在：

1、纯化抗体: 将这些蛋白偶联于支持物上，提供了纯化抗体的有效方法。
2、免疫沉淀(immunoprecipitation): 利用蛋白A、蛋白G和免疫球蛋白的Fc段的结合，把Ag-Ab复合物分离出来，从而达到从蛋白复合物中分离出特定蛋白进行下一步的研究分析或者研究蛋白质-蛋白质之间的相互作用。
3、去除或减少非特异性背景：如免疫沉淀技术中在加入特异性抗体前加protein A/G beads到细胞裂解液中，以减低背景。
蛋白A与不同的免疫球蛋白的结合能力并不一样，它们受到后者的来源及亚类的影响。

Protein A
Staphylococcal Protein A Recombinant 金黄色葡萄球菌A蛋白(Staphylococcal Protein A、简称SPA)

Catalog No. PRO-356

Synonyms:
Immunoglobulin G-binding protein A, IgG-binding protein A, Staphylococcal protein A, SPA.

Introduction:
Protein A is a cell wall protein deriving from Staphylococcus aureus which exhibits unique binding properties for IgG from a variety of mammalian species and for some IgM and IgA as well. It binds with the Fc region of immunoglobulins through interaction with the heavy chain. It couples to a wide variety of reporter molecules including fluorescent dyes, enzyme markers, biotin, colloidal gold and radioactive iodine without affecting the antibody binding site. Recombinant Protein A was developed to increase the specificity of the molecule for IgG and is widely used both in research and bioprocessing. The recombinant protein A is produced by expressing a modified protein A gene in E.coli. A specific purification process with strict quality control was taken to get the recombinant protein A with the purity of more than 98% , no human IgG affinity step is used during validated fermentation and purification and devoid of bacterial contaminant found normally in native Protein A. (Free of Staphylococcus endotoxins and hemolysin).

Description:
Recombinant Staphylococcal Protein A produced in E.Coli is a non-glycosylated, Polypeptide chain having a molecular mass of 45 kDa.
Recombinant Staphylococcal Protein A is purified by proprietary chromatographic techniques.

Source:
Escherichia Coli.

Physical Appearance:
Sterile Filtered clear colorless solution.

Formulation:
The protein solution contains no additives.

Stability:
SPA should be stored at -20°C.

Purity:
Greater than 98.0% as determined by RP-HPLC.

Activity:
Greater than 95.0% binding activity to human IgG.

Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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» Protein A, 434 a.a
» Protein A/G
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