重组小鼠胰岛素样生长因子-1 (rMuIGF-I) Recombinant Murine igf-1

Recombinant Murine IGF-I

【简介】

Synonyms:Insulin-like Growth Factor-I, Somatamedin C, IGF-IA

The IGFs are mitogenic polypeptide growth factors that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. IGFs are predominantly produced by the liver, although a variety of tissues produce the IGFs at distinctive times. The IGFs belong to the Insulin gene family, which also contains insulin and relaxin. The IGFs are similar by structure and function to insulin, but have a much higher growth-promoting activity than insulin. IGF-II expression is influenced by placenta lactogen, while IGF-I expression is regulated by growth hormone. Both IGF-I and IGF-II signal through the tyrosine kinase type I receptor (IGF-IR), but, IGF-II can also signal through the IGF-II/Mannose-6-phosphate receptor. Mature IGFs are generated by proteolytic processing of inactive precursor proteins, which contain N-terminal and C-terminal propeptide regions. Recombinant murine IGF-I is a 7.7 kDa globular protein containing amino acids including 3 intra-molecular disulfide bonds.

【表达系统】

Escherichia coli

【质量控制】

分子量:

Approximately 7.7 kDa, a single non-glycosylated polypeptide chain containing 70 amino acids.

内毒素:

Less than 1 EU/μg of IGF-I as determined by LAL method.

产品用途:

This material is offered by propbs Biotechnology for research, laboratory or further evaluation purposes. FOR RESEARCH USE ONLY. NOT INTENDED FOR ANY ANIMAL OR HUMAN THERAP EUTIC OR DIAGNOSTIC USE.

生物学活性:

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation

assay using serum free human MCF-7 cells is less than 2 ng/ml, corresponding to a specific activity

of > 5.0 × 10⁵ IU/mg.

产品形态:

Sterile filtered white lyophilized (freeze-dried) powder.

氨基酸序列:

GPETLCGAEL VDALQFVCGP RGFYFNKPTG YGSSIRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPTKAA

产品纯度:

> 98% by SDS-PAGE and HPLC analyses.

【成份】

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

【溶解方法】

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions.

【保存】

This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

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