重组大鼠碱性成纤维细胞生长因子（rRtbFGF）Recombinant Rat FGF-b

Recombinant Rat FGF-b

【简介】

Fibroblast Growth Factor-basic (bFGF) is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-basic is a non-glycosylated heparin binding growth factor that is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland liver, monocytes, epithelial cells and endothelial cells. FGF-basic signals through FGFR 1b, 1c, 2c, 3c and 4.

【表达系统】

Escherichia coli

【质量控制】

分子量:

Approximately 16.3 kDa, a single non-glycosylated polypeptide chain containing 146 amino acids.

内毒素:

Less than 1 EU/μg of bFGF as determined by LAL method.

产品用途:

This material is offered by propbs Biotechnology for research, laboratory or further evaluation purposes. FOR RESEARCH USE ONLY. NOT INTENDED FOR ANY ANIMAL OR HUMAN THERAP EUTIC OR DIAGNOSTIC USE.

生物学活性:

The ED50 determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 1.8 ng/mL, corresponding to a specific activity of > 5.6 × 10⁵ IU/mg.

产品形态:

Sterile filtered white lyophilized (freeze-dried) powder.

氨基酸序列:

MPALPEDGGG AFPPGHFKDP KRLYCKNGGF FLRIHPDGRV DGVREKSDPH VKLQLQAEER GVVSIKGVCA NRYLAMKEDG RLLASKCVTE ECFFFERLES NNYNTYRSRK YSSWYVALKR TGQYKLGSKT GPGQKAILFL PMSAKS

产品纯度:

> 98% by SDS-PAGE and HPLC analyses.

【成份】

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

【溶解方法】

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions.

【保存】

This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

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