重组大鼠血小板源性生长因子（rRtPDGF-BB）Recombinant Rat pdgf-bb

Recombinant Rat PDGF-BB

【简介】

Synonyms:

Platelet-Derived Growth Factor-BB, Glioma-derived growth factor (GDGF), Osteosarcoma-derived Growth Factor (ODGF)

Platelet-derived growth factor is a dimeric glycoprotein composed of two A (-AA) or two B (-BB) chains or a combination of the two (-AB). All PDGFs function as secreted, disulphide-linked homodimers, but only PDGFA and B can form functional heterodimers. PDGFs are mitogenic during early developmental stages, driving the proliferation of undifferentiated mesenchyme and some progenitor populations. PDGF is a required element in cellular division for fibroblasts, a type of connective tissue cell

that is especially prevalent in wound healing. In essence, the PDGFs allow a cell to skip the G1 checkpoints in order to divide. Recombinant rat PDGF-AA contains 109 amino acids and has a molecular mass of 12.2 kDa.

【表达系统】

Escherichia coli

【质量控制】

分子量:

Approximately 24.4 kDa, a disulfide-linked homodimeric protein containing two 109 amino acid residues polypeptide (B chain).

内毒素:

Less than 1 EU/μg of PDGF-BB as determined by LAL method.

产品用途:

This material is offered by propbs Biotechnology for research, laboratory or further evaluation purposes. FOR RESEARCH USE ONLY. NOT INTENDED FOR ANY ANIMAL OR HUMAN THERAP EUTIC OR DIAGNOSTIC USE.

生物学活性:

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation

assay using murine Balb/c 3T3 cells is less than 2.0 ng/ml, corresponding to a specific activity of > 5.0 × 10⁵ IU/mg.

产品形态:

Sterile filtered white lyophilized (freeze-dried) powder.

氨基酸序列:

SLGSLAAAEP AVIAECKTRT EVFQISRNLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RASQVQMRPV QVRKIEIVRK KPVFKKATVT LEDHLACKCE TVVTPRPVT

产品纯度:

> 98% by SDS-PAGE and HPLC analyses.

【成份】

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

【溶解方法】

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions.

【保存】

This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

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