Recombinant Human EGF

【简介】

Synonyms:Epidermal Growth Factor, Urogastrone, URG

Epidermal Growth Factor (EGF) was originally discovered in crude preparations of nerve growth factor prepared from mouse submaxillary glands as an activity that induced early eyelid opening, incisor eruption, hair growth inhibition, and stunting of growth when injected into newborn mice. Human EGF was isolated from urine based on its inhibitory effect on gastric secretion and named urogastrone, accordingly. EGF is prototypic of a family of growth factors that are derived from membrane-anchored precursors. All members of this family are characterized by the presence of at least one EGF structural unit (defined by the presence of a conserved 6 cysteine motif that forms three disulfide bonds) in their extracellular domain. EGF is initially synthesized as a 130 kDa precursor transmembrane protein containing 9 EGF units. The mature soluble EGF sequence corresponds to the EGF unit located proximal to the transmembrane domain. The membrane EGF precursor is capable of binding to the EGF receptor and was reported to be biologically active.

【表达系统】

Escherichia coli

【质量控制】

分子量:

Approximately 6.2 kDa, a single non-glycosylated polypeptide chain containing 53 amino acids.

内毒素:

Less than 1 EU/μg of EGF as determined by LAL method.

产品用途:

This material is offered by propbs Biotechnology for research, laboratory or further evaluation purposes. FOR RESEARCH USE ONLY. NOT INTENDED FOR ANY ANIMAL OR HUMAN THERAP EUTIC OR DIAGNOSTIC USE.

生物学活性:

The ED50 determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 0.1 ng/mL, corresponding to a specific activity of > 1.0 × 10⁷ IU/mg.

产品形态:

Sterile filtered white lyophilized (freeze-dried) powder.

氨基酸序列:

NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR

产品纯度:

> 98% by SDS-PAGE and HPLC analyses.

【成份】

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

【溶解方法】

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions.

【保存】

This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

针对不同客户的研究需要，提供的重组蛋白产品包括：生长因子、趋化因子、细胞因子以及各种活性蛋白。重组蛋白产品来源于不同的表达系统，包括植物、大肠杆菌、哺乳动物细胞和昆虫细胞等，可以满足不同的研究的需要。产品经过纯度、活性与内毒素检测，可以满足不同的应用需要。

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