重组人β-神经生长因子/Recombinant Human beta-NGF

Recombinant Human beta-NGF

【简介】

Synonyms: Beta Polypeptide, NGF-β,β-NGF,NGF, NGFB, HSAN5, Beta-NGF, MGC161426, MGC161428.

β-NGF is a neurotrophic factor structurally related to BDNF, NT-3 and NT-4. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. β-NGF is a potent neurotrophic factor that signals through its receptor β-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. β-NGF also acts as a growth and differentiation factor for B lymphocytes and enhances B-cell survival. The functional form of human β-NGF is a noncovalently disulfide-linked homodimer, of two 13.5 kDa polypeptide monomers (240 total amino acid residues). The three disulfide bonds are required for biological activity.

【说明】

Escherichia coli

【质量控制】

分子量:

Approximately 13.5 kDa, a single non-glycosylated polypeptide chain containing 120 amino acids.

内毒素:

Less than 1 EU/μg of β-NGF as determined by LAL method.

产品用途:

This material is offered by propbs Biotechnology for research, laboratory or further evaluation purposes. FOR RESEARCH USE ONLY. NOT INTENDED FOR ANY ANIMAL OR HUMAN THERAP EUTIC OR DIAGNOSTIC USE.

生物学活性:

Determined by the dose-dependent stimulation of the proliferation of human TF-1 cells.The expected ED50is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 10⁶units/mg.

产品形态:

Sterile filtered white lyophilized (freeze-dried) powder.

氨基酸序列:

MSSSHPIFHRGEFSVCDSVSVWVGDKTTATDIKGKEVMVLGEVNINNSVFKQYFFETKCRDPNPVDSGCRGIDSKHWNSYCTTTHTFVKALTMDGKQAAWRFIRIDTACVCVLSRKAVRRA.

产品纯度:

> 98% by SDS-PAGE and HPLC analyses.

【成份】

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

【溶解方法】

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions.

【保存】

This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

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